Trichoderma, also known as green mold, common species of Pleurotus eryngii is: Trichoderma viride and Trichoderma koningii, is a semi-known fungus subphylum, filamentous spores, caterpillars, plexiaceae, Trichoderma.
First, the harm symptoms
Trichoderma is the most serious species of bacteria that invades Pleurotus eryngii. Any suitable medium for the growth of Pleurotus eryngii is suitable for the growth of trichoderma. In the case of strains carrying Trichoderma or indiscriminate disinfection during inoculation and high concentrations of Trichoderma spores in the inoculation chamber, the inoculation surface falls into the trichoderma spores, and Trichoderma spores rapidly germinate and inoculate, covering the inoculation surface, making Pleurotus eryngii Hyphae loses nutrient supply and stops growing, resulting in failure to inoculate. During the fruiting period, the mushroom body was hindered from growing in adverse environments, and the resistance was reduced and it was easily infected with Trichoderma. After the mushroom body was infested, the growth, softening, and waterlogging were stopped, and the mushroom body was covered with Trichoderma mycelium.
Second, control methods
1. Keep the environment of the preparation and germination sites clean and dry, free of waste and accumulation of contaminated materials. The bagging workshop is isolated from the sterile room to prevent the dust from coming into contact with the sterilized growing bag during mixing.
2. The thickness of the cultivation bag is 0.5-0.55 mm, no micropores. When formulating the medium formulation, try not to add or add less sugar. The moisture in the medium is controlled at 60% to 65%. Excessive moisture can easily cause Trichoderma breeding.
3. Thoroughly sterilize, prevent cooling and non-uniform thermal cycling in the sterilization process. Atmospheric sterilization requires 100°C for 10 hours. Autoclaving requires 121°C-126°C for 2.5 hours.
4. Seal cooling, timely inoculation, appropriate increase in inoculation. Guarantee the purity and vitality of Pleurotus eryngii. Ensure that the inoculation room and the inoculation box are highly sterile, can effectively reduce the pollution rate during the inoculation process, the inoculation room should be provided with a buffer room, and sterilization and sterilization should be performed before the cultivation bag enters.
5. Low temperature inoculation, constant temperature bacteria. Strengthen the germination period inspection and found that the contaminated bacteria bags need to be shipped out in time to reduce the chance of repeated contamination.
6. Keep mushrooming place health, mushroom room to maintain ventilation, appropriate to reduce the air humidity, reduce the number of water spray, timely mushroom, remove the mushroom, root and mushroom, remove the pollution bacteria bags.
step 2: Immediately close the container again.
This protects the remaining test strips from humidity and guarantees strip integrity up to the expiry date indicated.
step 3: Dip the test strip for about 1 second into the fresh urine specimen.
Wipe off any excess urine on the rim of the vessel and blot the edge of the test strip on tissue paper.
step 4: Read the result by comparing the test fields with the color scale on the test pack after 30 to 60 seconds.
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