Nicotinamide adenine dinucleotide phosphate (NADP+) is called reduced coenzyme II, the scientific name reduced nicotinamide adenine dinucleotide phosphate, once known as pyridine nucleotide triphosphate, English triphosphopyridine nucleotide, using the abbreviation TPN, Also written as [H], also known as reducing hydrogen. N refers to nicotinamide, A refers to adenine, D 
It is a dinucleotide and P is a phosphate group. The role of hydrogen donors in chemical reactions in many organisms is of great significance. It is a phosphorylated derivative of the 2'-position of the ribose ring system linked to adenine in nicotinamide adenine dinucleotide (NAD+) and is involved in a variety of anabolic reactions such as lipids, fatty acids and nucleotides. . In these reactions, NADPH is required as a reducing agent, a donor of hydride ions, and NADPH is a reduced form of NADP+.
As a coenzyme, NADP is involved in the redox reaction and electron transfer essential for normal cell life. The NADP/NADPH ratio in a normal organism is always in a state of dynamic equilibrium. This ratio is a very important indicator that reflects the redox state of a cell, thereby determining the metabolic activity of a cell and the health of the cell. In healthy mammalian tissues, the ratio of NADP/NADPH is typically 0.005.
As a technology company dedicated to providing high-end products, technical support and services to high-end customers such as scientific research institutions and universities, Amy Jet introduces you to the NADP/NADPH ratio and quantitative analysis of the hotspots of cell metabolism research, including Convenient colorimetric assays and more sensitive fluorescence assays for different complete research protocols to meet the experimental conditions of your customers.
The Amplite Fluorimetric NADP/NADPH Ratio Assay Kit (Cat. No. AAT-15264) is a fluorescent detection NADP/NADPH ratio detection kit with high sensitivity. This detection method can significantly reduce interference from the biological sample itself because it is in the red visible light range. The detection method shows high sensitivity and low interference, excitation at 570 nm, emission at 590 nm, and detection of NADP, NADPH and NADP/NADPH ratios respectively; NADP/NADPH Quantitation Colorimetric Kit (Cat. No. K347-100) It is a colorimetric detection kit, which is convenient for most laboratories and easy to operate and fast. Both of the above recommended kits are capable of detecting NADP, NADH and NADP/NADPH ratios for an unbeatable price/performance ratio. These two kits are highly specific and do not cross-react with NADP, absolute value!
In addition, for some experiments that require separate detection of NADPH content, Aimei also recommends a separate test kit for NADPH and total NADP/NADPH. It is also suitable for colorimetric and fluorescent methods. Choose the right test solution according to your needs.
In addition to the NADP/NADPH Quantitative and Ratio Analysis Kit, Amy Jie also recommends other commercial kit product solutions, including NAD/NADH quantification and ratio analysis, as well as other glucose metabolism, glycolysis pathways, and acetone. Related analysis kits for acid metabolism, lactic acid metabolism and other cellular metabolism, to help customers research and fuel!
Amy Jie can find and provide the best product solutions for you! Including a variety of detection and analysis kits, related antibodies, recombinant proteins and cytokines and enzymes, as well as other innovative research tools. Its products cover areas such as apoptosis research, energy metabolism, cell/tissue protein extraction, oxidative stress and injury, metabolism and obesity, cell proliferation and toxicity, signal transduction, and stem cell research. Amy is committed to providing the best and most up-to-date research tools in cell biology to continuously expand the quality of its products and services. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.
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