Precautions for gel recovery and gel imaging during electrophoresis

Precautions for gel recovery and gel removal during electrophoresis:

1. The electrophoresis buffer and gel are all made freshly. The table for cutting the glue is cleaned, and the blade is best cleaned and sterilized. One of the words is to ensure that the cut tape has no foreign DNA pollution.

2. The glue is to recover all the glue in the position of the entire target piece. In order to reduce the volume of the glue, it can be done with a relatively thin glue, as long as it is enough to be spotted, a thin and wide comb can also be used to run the glue.

3. Regarding the protection, it is enough after the general plexiglass, put on a protective mask to protect the eyes, if you wear resin glasses. If you are very afraid of UV radiation, or have special requirements for glue, such as the requirement for EB-free, you can use a dot marker and a graduated ruler to take a photo to determine the position of the target strip on the glue.

4. Follow the normal procedure to mark the glue, then cut the glue with the marker, EB stain, and take photos with the graduated ruler under the UV lamp. Since the relative position between the belt to be recovered and the marker is known, the position of the target belt on the undyed glue can be measured by the ruler. If you find it difficult to judge, you can point a small amount directly on the marker, so the position is easy to determine.

Use and precautions for gel imaging analysis systems:

Gel imaging analysis system: It can be applied to protein electrophoresis gel, DNA gel, sample for image acquisition and qualitative and quantitative analysis. Samples include: EB, SYBR Green, SYBR Gold, Texas Red, GelStar, Fluoroscecin, Radiant Red Equally stained nucleic acid monitoring; and Coomassie Blue, SYPRO Orange, various stained protein gels such as test dyes. (or UV, EB and colored and visible sample imaging)

Precautions for use:
• Pay attention to the boot sequence, first open the gel imaging system, then turn on the computer to enter the software.
• To prevent EB contamination of the instrument during UV gel photography, the door of the gel imaging system should not be in contact with contaminated gloves and should not be exposed to contaminated gloves during software operation.
• The front panel of the gel imaging system cannot be opened during photography with an ultraviolet light source.
• Remove the waste material after taking the picture and wipe it off with a soft paper.
• Be light when focusing, and do not be drastic.

• Environmental voltage instability, use the power supply. In case of power failure during use, please turn off the power of the instrument until the call is re-initiated.

• When using, turn on the power switch of the instrument, then turn on the computer switch and open the software ( Quantity One ).

• Keep the observation room dry and promptly drain the water or other liquids left on the observation board (use soft paper, usually roll paper).

• Be careful not to contaminate the surface of the instrument when observing gels stained with EB (ethyl bromide). Never touch the gel directly with your hands, or wear gloves that have been in contact with the gel to access the door of the instrument and the handle of the observatory.

• When using the instrument, close the door and the observatory, otherwise the UV lamp will not work properly.

• Try not to connect your computer to the Internet or a local area network, and install anti-virus software on your computer (recommended to use genuine software).

• When the instrument is not used for a long time, cover the instrument with a dust cover.

• To extend the life of the lamp, turn off the light source after observing the gel (the instrument itself has a 15-minute automatic protection program).