Introduction to the formula of Cordyceps sinensis

Introduction to the formula of Cordyceps sinensis


Cordyceps sinensis is a fungus that mainly parasitizes living organisms to decompose carbon sources such as organic matter, nitrogen sources and inorganic salts. This type of nutrient must be properly formulated during artificial cultivation to meet its growth requirements. The following raw materials and formulas for the growth of Cordyceps sinensis mycelium are screened as follows:

First, the mother plant production medium formula:

1. Ma Ling potato 200 g (cooked juice), glucose 20 g, agar 20 g, potassium dihydrogen phosphate 1 g, magnesium sulfate 1 g, peptone 1 g, beef extract 0.5 g, water 1000 ml.

2, protein 胨 1 g, glucose 20 g, yeast extract 1 g, potassium dihydrogen phosphate 1 g, magnesium sulfate 1 g, VB11 g, VB121 g, agar 20 g, water 1000 ml.

3, bean sprouts 500 grams (cooked juice), sugar 20 grams, 20 grams of agar, potassium dihydrogen phosphate 1 g, magnesium sulfate 1 g, peptone 0.5 g, water 1000 ml. One of the above formulas may be selected and processed according to other parent species in the conventional manner, and dispensed in 1/5 of a 180×18 test tube, and autoclaved at 1.5 lbs for 30 minutes to prepare a slant medium.

Inoculation: After the residual water on the surface of the slanted medium is dried, it is inoculated into a sterile inoculation box, and the separated pure Cordyceps sinensis mother seed is picked up and placed on a slant medium, and each mother can be connected to 10 to 15 pieces. However, the passage of the mother species does not exceed two times, otherwise the infection will be lost.

Culture: After inoculation, place it at a constant temperature of 15~18 °C. After three days, the mycelium began to germinate, and the yellowish and vigorous fluffy hyphae spread around the vine. Seven days later, the surface of the mycelium produced a sub-seat (ie, grass). Although this grass has no host of insects, its nutrients and Cordyceps sinensis. It is completely consistent. Under normal conditions, the mycelium can be overgrown with slant medium for about 10 days.

Second, liquid seed production medium formula:

1. Rice soup (rice cooked to semi-cooked filter) 500ml, glucose 5g, potassium dihydrogen phosphate 1g, magnesium sulfate 1g, peptone 1g, beef extract 1g, VB11g, VB121g.

2, noodle soup (cooked noodles soup) 500ml, sugar 5 grams, 1 g of peptone, 1 g of beef extract, 1 g of magnesium sulfate, 1 g of yeast extract, 1 g of potassium dihydrogen phosphate.

3. 500 ml of clear spring water, 10 g of glucose, 2 g of peptone, 2 g of beef extract, 2 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate, 12 g of VB, and 122 g of VB. The prepared nutrient solution was placed in a 500 ml flask, 150 ml per bottle, and the tampon was stoppered and autoclaved for 1.5 lbs for 30 minutes. Inoculation culture: After cooling, put it into the inoculation box for aseptic operation, take the large part of the mother seed medium into the liquid bottle, each mother can receive 5-10 bottles, and set it at 20~25°C. Set the culture for three days, then put it into the shaker at the same temperature and shake it. If there is no shaker, shake the liquid bottle 3-5 times a day for 3-5 minutes. After 10-15 days of cultivation, it will become Liquid species. If the cultivation amount is large and the liquid species is insufficient, the liquid species may be expanded to 2-3 times (grade) according to the production amount, and any liquid species may be used for directly cultivating Cordyceps sinensis, but the parent species or liquid Too many passages will affect the effect. Liquid species should not be stored for too long, generally in low temperature or dark conditions for less than one month. The effect is better. Zui is good to make liquid species before the cultivation season.

Verify that the liquid species is cultured successfully:

The method is that after 10-15 days of constant temperature and vibration culture, there is no obvious change from the color. Only after three days of stopping the vibration, the surface of the liquid gradually appears yellow-white hyphae without any abnormal color, which proves that the liquid species has been cultured successfully. Over time, a thick layer of fungus is formed on the surface of the liquid, and it is necessary to remove the excessively thick bacterial skin utilization supernatant on the surface during cultivation.

Third, the production of solid strains

Cordyceps sinensis is generally cultivated using liquid strains. Solid strains can also be used to test whether Cordyceps can grow. Cordyceps can also be directly grown in solid medium or PDA medium. It can be used on solid medium such as rice or silkworm. The stalk can be grown on the appropriate insect body by spraying liquid species onto the appropriate insect body.

Medium formula

1. The rice is boiled to a half-life filter, filled into canned bottles, and sealed with kraft paper or plastic film.

2, live corn or live wheat directly into the canned bottle (some products must be soaked for more than 12 hours) sealed and sterilized.

3, cotton shell 95%, rice flour 3%, soy flour 2%, water content 60%, bottling sterilization. The cells were incubated at 1.5 lbs. pressure for 60 minutes, or at atmospheric pressure for 100-6 C for 5-6 hours.

Inoculation: Sterile operation in the inoculation box, solid solid medium can be accessed regardless of the solid parent or liquid species. The mother can pick a large piece of the finger on the surface of the solid medium, or use the inoculation needle to pick up the hyphae and stir it evenly in the solid medium. Inoculation of liquid seeds is carried out by infiltrating the seed solution into a solid medium, and the amount of each bottle is not more than 5 ml.

Culture: Solid seeds should be cultured under dark light conditions of 15~18 °C. It takes only 15-20 days for the hyphae to grow over the bottle, and it takes one month for the mycelium to grow up with the solid mother seed. bottle.

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