Liquid chromatography is widely used as a sophisticated chemical analysis instrument, and its design and production covers precision machinery, microelectronics, software design, intelligent control and chemical analysis. When choosing a grating monochromator for high performance liquid chromatography instruments, special attention should be paid to the following principles:
Grating selection: monochromator design, the first consideration is designed in accordance with the high performance liquid chromatograph's category. If you are designing a UVD , you should first analyze the role of the grating in the UVD .
2 Type selection of monochromators: The type of monochromator is related to stray light. Stray light is one of the most important performance specifications in monochromators, so be careful when choosing a monochromator type.
3 slit design: From the theory of optical instrumentation, the line of the UVD monochromator of the high performance liquid chromatograph is curved on the slit spectrum, and the slit has a height problem, so the design slit Consider the spectral line bending problem.
4 Objective lens and collimator lens focal length design: the focal length of the monochromator and the focal length of the objective lens are related to the resolution of the monochromator. In general, the higher the resolution of the monochromator, the longer the focal length of the objective lens and collimator lens is required.
5 The relative aperture design of the focal length of the objective lens and the collimating mirror: the relevant designers generally advocate the use of a large liquid chromatograph objective lens and a collimating mirror aperture.
6 choice of blaze wavelength position: Generally, the position of the blazed wavelength is selected according to the requirements of different types of liquid chromatography instruments. HPLC UVD majority use, should be selected in the blaze wavelength ultraviolet region, it should generally be in place of 240 ~ 260nm.
7 Flatness selection of grating output energy: In the wavelength range of the grating, the output energy of other wavelengths other than the blazed wavelength should be as large as possible.
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