First, the rootstock culture 1, scaffolding. In the early spring, the weather is changeable and the middle shed can effectively regulate the temperature and humidity. Use a bamboo strip to take a scaffold that is 5 meters wide and about 2 meters high at the top of the arc. Put on 8 to 8 meters wide light-transmitting agricultural film to increase the temperature of the greenhouse 7-8 days before planting. The vertical section of the shed starts at 3 cars and each car is 1.3 meters wide. 2, nutrition soil preparation. Use rice field and fire soil in the former half and half, add 2 kg of compound fertilizer (15% for N, P, K) per hectare, mix and sieve after bagging of superphosphate (1.5 kg), and bag (the nutrition bag size can be used Cm, height 8 cm). Neatly placed on the surface of the box, sprayed the soil bag with 40% formalin 1000 times, and the film was suffocated for 3 days and the film was removed to remove the smell. 3, sowing. From the end of February to the beginning of March, sowing can be done, and a generation of hybrid four-season pods with good resistance and good symbiosis is selected as the rootstock seedlings. Seed drying for 3 hours before sowing, soaking for 24 hours, draining and placing in the environment of 28-32°C. Seed “whitening†(usually after 24 hours) sowing seeds, soaking nutritive soil before sowing, first using branches when sowing One hole is drilled, and the seed is placed flat in the hole and compacted. Finally, the cover is sealed and kept warm. 4, water and fertilizer management. After emergence, the bag soil will keep dry and see wet state. The high temperature in sunny days should be ventilated and cooled in time. Keep the temperature in the shed within 25-28°C during the day and 14-15°C during the night to avoid the occurrence of “tall shootsâ€. Second, scion culture 1, selection of species. According to experience, choose a good symbiotic good breed. 2, sowing germination. To be unearthed from the cotyledon of the rootstock (7 days after sowing of the general anvil seedlings). Wash the seed with water 3-5 times, remove the surface of the seed mucus, with hot water 50-60 °C for half an hour, then soaking for 6-8 hours, picked up after germination at 28-32 °C environment, after breaking Spread evenly on a pure river sand bed, and then cover it with 1 cm thick fine sand. Adjust the temperature and humidity as the rootstock seedlings are cultivated. Normally, the cotyledon flat and true leaf first grafting specifications can be achieved within 5-6 days. Third, graft preparation 1, grafting equipment. Razor blade, wedge-shaped bamboo skewer (port width of about 1.5-2 mm, not to break the hypocotyl limit), film, shading net. 2, before handling. After the first day, 70% thiophanate-methyl (or Taisheng M-45) was used to spray the rootstock seedlings and scion seedlings 800 times. If the weather is hot and dry during the adaptation period, the rootstock should be soaked with clean water before the day. Sand bed. Fourth, grafting method using the top plug. The left hand thumb and forefinger hold the cotyledonary node, the right hand holding a bamboo stick shovel to the true root and growth point of the rootstock, and insert a 1cm deep hole slightly obliquely downward from the wound near the end of the cotyledon, do not break through and penetrate the hypocotyl ( Avoid scion "self-rooted". Don't use bamboo sticks immediately. Take a vigorous scion immediately and place a knife 0.5 cm below the cotyledon section at the hypocotyl. Bevel into the cone of 1 cm noodle toward the root. Place the bamboo stick to scion quickly. The hypocotyls were inserted obliquely downwards into the holes, and the cotyledons of the scions and the cotyledons of the rootstocks were overlapped. Each received tree should be immediately placed in a closed shed and shaded to reduce water loss. Fifth, then management 1, temperature management. After that, the temperature in the shed is maintained at about 30°C during the day and 20°C at night to adjust the amount of ventilation in the shed so that the daily temperature in the seedbed is 25-28°C and the night temperature is 13-15°C. 2, humidity management. 3-4 days later, the high humidity in the shed must be maintained, and the relative humidity in the shed should be as high as 95%-100% through the compact size shed. From the 5th day, the ventilation gradually reduces the humidity and maintains the normal relative humidity after survival. About 65%-75%. 3, light control. On the day of grafting, shading is not visible, and light can be seen in the appropriate amount in the morning and evening of the second day. Afterwards, the amount of light can be gradually increased, and the scion is not wilting. Normal management can be shady after 7 days. 4, other management. Combine ventilation with the axillary buds growing on the rootstock. Ventilation volume should be from small to large. Membrane ventilation must be removed from the lee side. Generally, ventilation should be started at 9-10 a.m. in sunny days. Do not ventilate it when high temperatures do not occur at noon. seedling". Timely prevention and control of pests and diseases, mainly leafflies and damping-off disease, in addition to the use of pesticides, timely cutting off the cotyledons with diseases and insects.
Surgical Isolation Gown,Waterproof Isolation Gown,Disposable Surgical Gown,Sterile Surgical Gown
Ningbo Carest Medical Instrument Co.,ltd , https://www.carestmed.com