QSight Triple Quadrupole LC/MS is used to detect acrylamide in coffee

QSight, which is resistant to dirt , let us rest assured that a cup of coffee will not be worth the spring! (See the bottom of the benefits )

Spring blossoms, everything recovers, and it is full of vitality. Such a beautiful view, Xiao P about a friend Xiao E stepped on the footsteps of spring, pleasing the gentle sunshine, stroking the gentle spring breeze, kissing the overflowing floral fragrance, to enjoy the small life of life.

Xiao E is a member of the food testing laboratory. When Xiao E saw the small P, he poured out the recent bitter water. In order to cope with the increasing business volume, Xiao E recently worked overtime every day. In addition to eating and sleeping, he spent the rest of his time in the lab (fortunately, he has a girlfriend, otherwise he will be a bachelor in this state). In the past two days, the incident that coffee may contain carcinogen-acrylamide caused a panic, which led to a lot of tests for the detection of acrylamide in coffee. The leader urged me to take a break and hurry to finish the batch. Due to the complexity of the coffee matrix, HPLC, GC/MS usually requires pretreatment steps such as extraction, derivatization, purification, etc., which is complicated and time consuming. Although the LC/MS/MS method does not require the derivatization of acrylamide, it still needs After the SPE column is processed, facing a huge sample volume, it will be a round of overtime work. I am saddened to think about it.

After listening to the complaints of Xiao E, Xiao P said slowly, do you know PerkinElmer's QSight triple quadrupole LC/MS? With super high sensitivity and no fear of being dirty, no matter how complex the sample matrix is, how difficult it is to detect the challenge, and easily meet the challenge and get satisfactory results quickly. EU Directive (EU) 2017/2158 (to be implemented on April 11, 2018), specifies the limited level of acrylamide: roasted coffee: 400μg/kg, instant coffee: 850μg/kg, coffee substitute: 500-4000 Gg/kg. QSight is not afraid of dirty characteristics. For the detection of acrylamide in coffee, it does not require complicated pre-treatment, direct extraction, and can be sampled and analyzed, and meets the testing requirements, greatly improving work efficiency.

Is there any doubt that the sample with such a complicated coffee matrix is ​​directly extracted for injection analysis, and the reproducibility of the instrument is good? Can the measured data be seen?

Next, we speak with specific solutions and data:

Sample preparation method : accurately weigh 1 g of sample (accurate to 0.001 g), add 50 μL of 0.1 mg/mL ¹³ C ₃ -acrylamide internal standard working solution, add 50 mL of ultrapure water, shake for 10 min, Centrifuge at 8000 r/m for 5 min, take the supernatant to 1 mL, dilute to 10 mL, mix, and pass the 0.22 μm micropore filter to the injection vial for LC-MS/MS.

LC-MS/MS instrument method:

1. Liquid chromatography parameters: liquid chromatograph: PerkinElmer UHPLC system, the specific parameters are shown in Table 1.

Table 1 liquid chromatograph parameter table

2. Mass spectrometry parameters:

The following parameters PerkinElmer QSight 220 ^TM triple quadrupole mass spectrometer, for example, the title compound MS parameters shown in Table 2 and Table 3.

Table 2 list of compound mass spectrometry parameters

Table 3 mass spectrometry ion source parameters

A good chromatographic retention of acrylamide was obtained using a PerkinElmer AQ C18 column. An extracted ion chromatogram at a concentration of 0.5 μg/L is shown in Figure 1.

Figure 1 Extracted ion chromatogram of acrylamide at a concentration of 0.5 μg/L

Figure 2 acrylamide standard curve, concentration range: 0.2 μg / L -100.0 μg / L

Under the optimized experimental conditions, the coffee sample was added to the standard recovery experiment. The recovery rate of acrylamide in the coffee sample was 73.7%-90.5%, and the relative standard deviation RSD value was 1.4-3.8%. The experimental results showed the recovery rate of the method and Reproducibility meets the analytical requirements for acrylamide in coffee.

Conclusion: This protocol establishes a rapid, highly sensitive and reliable LC-MS/MS method for the determination of acrylamide in coffee. It has good linearity (R ² ≥0.998) in the range of 0.2-100 μg/L. The method has simple pretreatment, high sensitivity, good recovery rate and excellent reproducibility, and fully meets the daily testing requirements of acrylamide in coffee and other foods.

After listening to Xiao E, he said that if the worker wants to do something good, he must first sharpen his tools. Personal ability is very important. The efficiency of the instrument is more important. It is time to find a leader to communicate with the instrument, so that there will be more time to enjoy this. Good scenery.

Welfare is coming! Do you have the same difficulties as the small E? Do you want to experience the QSight that is not afraid of dirty? Come and scan the code, we will arrange a special person to communicate with you and provide the QSight triple quadrupole liquid chromatography instrument. Once confirmed, it is also intended to send customers a beautiful gift - Xiaomi ultra-thin charging treasure .

About PerkinElmer:

As the world's leading provider of scientific instruments and services, PerkinElmer is committed to creating a healthier world. Our business covers medical diagnostics, scientific research and analytical instruments. With 11,000 professional technicians around the world, we are always ready to provide the best service to our customers and help them solve their scientific problems. Our expertise in analytical testing, medical imaging, information technology and after-sales services, as well as in-depth market insights, helps our clients to explore and improve our living environment. In 2017, PerkinElmer received $2.3 billion and served more than 150 countries and territories. It is a member of the Standard & Poor's 500 Index and listed on the New York Stock Exchange under the symbol 1-877-PKI-NYSE. To learn more about PerkinElmer, please visit PerkinElmer's official website at