First, P2 laboratory
The P2 laboratory is the secondary biosafety laboratory, which is equivalent to the BSL-2 laboratory.
The BLS-2 laboratory is mainly used for primary health services, diagnosis and research. The hazard level of the subjects is Grade II (medium individual hazard, limited group hazard), specifically defined as “can cause human or animal disease, but in general A pathogen that does not cause serious harm to health workers, groups, livestock or the environment. Laboratory infections do not cause serious illness, have effective treatment and preventive measures, and have limited transmission risks."
The BLS-2 laboratory specifies the following technical indicators (static):
1, cleanliness: no requirements
2, the minimum number of air changes: can open the window ventilation
3. Differential pressure in the room adjacent to the outdoor direction: no requirement
4, temperature °C: 18~27
5, relative humidity%: 30~70
6, noise dB (A): ≤ 60
7, the minimum illumination lx: 300
Second, construction, structure and decoration requirements
Most of the BLS-2 laboratories are rebuilt in the original building, unlike the newly built BLS-2 laboratory (in the national standard, the new BLS-2 laboratory is required to leave the public place for a distance), and other adjacent rooms. There is a relative isolation problem between them.
Therefore, in the original building to rebuild the BLS-2 laboratory, we should pay attention to the following issues.
1. The BLS-2 laboratory should be built in a shared building. A locked door that can be automatically closed should be provided. If necessary, a buffer zone, such as a buffer room, can be set up.
2. If there is no mechanical ventilation system, there should be windows for natural ventilation and insect screens. (In general, there should be a mechanical ventilation system, otherwise the temperature and humidity indicators are difficult to guarantee).
3, there should be measures to prevent insects, rats and other animals from entering and fleeing.
Third, air conditioning, ventilation and purification
In order to meet the room temperature and humidity requirements of the BLS-2 experiment, it is advisable to equip the mechanical air conditioning ventilation system. If there are special requirements, additional design requirements can be added, such as adding purification requirements and designing the air supply and exhaust system according to the purification requirements.
1. An air conditioning system with circulating air can be used. If chemical solvents, infectious material handling and animal experiments are involved, a full exhaust system should be used.
2. The biological safety cabinet is connected to the exhaust system.
The I-class exhaust ratio is 100%, and the connection is closed.
The Class II A type has a 30% exhaust ratio and can be discharged to the room or set up with a partial exhaust hood.
The B1 type exhaust air ratio is 70%, and it is hermetically connected.
The B2 type exhaust air ratio is 100% and is hermetically connected.
3. The location of various equipment in the laboratory should facilitate the flow of air from the “clean†space to the “contaminated†space, minimizing indoor backflow and leakage (biological safety cabinets should generally be placed at the most downstream of the indoor airflow, ie farthest away At the air supply outlet).
* Exhaust air from the biosafety cabinet is not a substitute for indoor exhaust.
Fourth, drainage
The bacteria-containing sewage should be discharged to the municipal sewage discharge public system after high temperature and high pressure sterilization or chemical disinfection.
Five, electrical settings
1. A dedicated distribution box should be provided.
2. The power supply should be equipped with a leakage detection and alarm device.
3, there is a reliable power system, the grounding resistance should not be greater than 1Ω
4, the laboratory entrance should have a textual light signal display in the laboratory working state.
5. Emergency light evacuation indication signs should be provided.
Sixth, fire
The fire resistance rating of the building is not less than two.
Seven, the equipment should be equipped with biological safety cabinet
(1) Class I biological safety cabinets may be used to generate microbial aerosols or to cause spills.
(2) For the treatment of inductive materials, a Class II biological safety cabinet should be used;
(3) For the treatment of chemical carcinogens, radioactive materials and volatile solvents, Class II-B biological safety cabinets should be used.
High temperature and high pressure sterilization disinfection cabinet.
Eye washers, emergency sprinklers should be provided if necessary.
The ultra-clean workbench is not a biosafety cabinet and cannot be used for biosafety operations.
The BLS-2 laboratory is used for preliminary biosafety experiments, and the national standard specifies the corresponding requirements.
The construction objectives are determined on the premise of ensuring safety, and the established laboratory should be a safe and economic laboratory.
The P2 laboratory is the secondary biosafety laboratory, which is equivalent to the BSL-2 laboratory.
The BLS-2 laboratory is mainly used for primary health services, diagnosis and research. The hazard level of the subjects is Grade II (medium individual hazard, limited group hazard), specifically defined as “can cause human or animal disease, but in general A pathogen that does not cause serious harm to health workers, groups, livestock or the environment. Laboratory infections do not cause serious illness, have effective treatment and preventive measures, and have limited transmission risks."
The BLS-2 laboratory specifies the following technical indicators (static):
1, cleanliness: no requirements
2, the minimum number of air changes: can open the window ventilation
3. Differential pressure in the room adjacent to the outdoor direction: no requirement
4, temperature °C: 18~27
5, relative humidity%: 30~70
6, noise dB (A): ≤ 60
7, the minimum illumination lx: 300
Second, construction, structure and decoration requirements
Most of the BLS-2 laboratories are rebuilt in the original building, unlike the newly built BLS-2 laboratory (in the national standard, the new BLS-2 laboratory is required to leave the public place for a distance), and other adjacent rooms. There is a relative isolation problem between them.
Therefore, in the original building to rebuild the BLS-2 laboratory, we should pay attention to the following issues.
1. The BLS-2 laboratory should be built in a shared building. A locked door that can be automatically closed should be provided. If necessary, a buffer zone, such as a buffer room, can be set up.
2. If there is no mechanical ventilation system, there should be windows for natural ventilation and insect screens. (In general, there should be a mechanical ventilation system, otherwise the temperature and humidity indicators are difficult to guarantee).
3, there should be measures to prevent insects, rats and other animals from entering and fleeing.
Third, air conditioning, ventilation and purification
In order to meet the room temperature and humidity requirements of the BLS-2 experiment, it is advisable to equip the mechanical air conditioning ventilation system. If there are special requirements, additional design requirements can be added, such as adding purification requirements and designing the air supply and exhaust system according to the purification requirements.
1. An air conditioning system with circulating air can be used. If chemical solvents, infectious material handling and animal experiments are involved, a full exhaust system should be used.
2. The biological safety cabinet is connected to the exhaust system.
The I-class exhaust ratio is 100%, and the connection is closed.
The Class II A type has a 30% exhaust ratio and can be discharged to the room or set up with a partial exhaust hood.
The B1 type exhaust air ratio is 70%, and it is hermetically connected.
The B2 type exhaust air ratio is 100% and is hermetically connected.
3. The location of various equipment in the laboratory should facilitate the flow of air from the “clean†space to the “contaminated†space, minimizing indoor backflow and leakage (biological safety cabinets should generally be placed at the most downstream of the indoor airflow, ie farthest away At the air supply outlet).
* Exhaust air from the biosafety cabinet is not a substitute for indoor exhaust.
Fourth, drainage
The bacteria-containing sewage should be discharged to the municipal sewage discharge public system after high temperature and high pressure sterilization or chemical disinfection.
Five, electrical settings
1. A dedicated distribution box should be provided.
2. The power supply should be equipped with a leakage detection and alarm device.
3, there is a reliable power system, the grounding resistance should not be greater than 1Ω
4, the laboratory entrance should have a textual light signal display in the laboratory working state.
5. Emergency light evacuation indication signs should be provided.
Sixth, fire
The fire resistance rating of the building is not less than two.
Seven, the equipment should be equipped with biological safety cabinet
(1) Class I biological safety cabinets may be used to generate microbial aerosols or to cause spills.
(2) For the treatment of inductive materials, a Class II biological safety cabinet should be used;
(3) For the treatment of chemical carcinogens, radioactive materials and volatile solvents, Class II-B biological safety cabinets should be used.
High temperature and high pressure sterilization disinfection cabinet.
Eye washers, emergency sprinklers should be provided if necessary.
The ultra-clean workbench is not a biosafety cabinet and cannot be used for biosafety operations.
The BLS-2 laboratory is used for preliminary biosafety experiments, and the national standard specifies the corresponding requirements.
The construction objectives are determined on the premise of ensuring safety, and the established laboratory should be a safe and economic laboratory.
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