The configuration and production of the medium are all painstakingly obtained through layers. For professional and first-class researchers, the storage of the medium has a self-evident significance for the evaluation of services such as the medium. . If the culture of word-of-mouth is not properly managed, it will be a very painful thing. The following is a summary of the storage considerations for the culture media combined with the practical experience of the authoritative experimenter.
What are the precautions for storage medium?
First: create unique preservation conditions
The medium-quality and well-recognized medium label should be marked with the expiration date and the relevant characteristics of the medium. Manufacturers and users can create unique storage conditions based on their own conditions. In addition, the transportation conditions used must also be controlled to a minimum of moisture loss and provide mechanical protection.
Second: adjust the reliable and suitable temperature
If the medium is stored in an autoclave after sterilization, the quality may be affected, and most senior laboratories do not advocate this storage method. For example, agar media should not be stored at or below zero, as freezing may damage the gel properties.
Third: create a high-quality light-proof sealed environment
The medium should be stored away from light. If it is to be stored for a long time, it should be placed in a closed container to prevent water loss. Agar plates are more suitable for use now, of course, they can also be stored in the freezer, usually within a week. If the preservation is extended, the shelf life needs to be verified.
Fourth: do a good job of scientific antifouling measures
The remelting of the solid medium after sterilization is allowed only once to avoid deterioration of the medium quality or microbial contamination due to excessive heat. The remelting of the medium is generally carried out using a water bath to heat or circulate the steam. The used medium (including the failed medium) should be disposed of in accordance with the relevant regulations on national waste disposal.
In summary, the storage of the medium as a very easy to be overlooked part of the laboratory is a lot of details worthy of attention. Senior scholars with experience and demeanor have a clear understanding of the importance of this step. I hope that the majority of scientific research personnel will make four unique aspects of storage in a rigorous and pragmatic manner, and make their own unique contributions to scientific research.
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